wen0715

March 13, 2014

University cheap beats by dre solo hd of S Paulo

Alpha and vegf expression by sp1 decoy oligodeoxynucleotides in mouse melanoma tumor http://www.teamskyuser.co.uk/beats-by-dr-dre-pro.html Downregulation of tnf and vegf expression by sp1 drebtshdphones decoy oligodeoxynucleotides in mouse melanoma tumor E m novak1, m metzger2, 3, r chammas4, 5, m da costa4, k dantas1, c manabe1, j pires1, a c de oliveira1 and s p bydlowski1, 6 1Research and Molecular Biology Division, Pr Hemocentro de S Paulo Foundation, S Paulo, Brazil2Heart Institute, University of S Paulo School of Medicine, S Paulo, Brazil3Department of Physiology and Biophysics, Institute of Biomedical Sciences, University cheap beats by dre solo hd of S Paulo, S Paulo, Brazil4Laborat de Oncologia Experimental, Depto.De radiologia, faculdade de medicina da universidade de s paulo, brazil5center for research on cellbased therapy, ribeir preto, brazil6department of hematology, university of s paulo school of medicine, s paulo, brazilcorrespondence r em novak, pr hemocentro de s paulo foundation, research and molecular biology division, av.Dr en de carvalho aguiar, 1551 andarbl.12, 05403000, S Paulo, SP, Brazil. Top of pageabstractmelanoma tumor growth and progression are highly dependent on adequate blood supply through angiogenesis.Since several genes involved in angiogenesis revealed potential binding sites for the transcription factor sp1, we have examined the effects of local inoculation of sp1 decoy oligodeoxynucleotides(Odns)On the growth of transplanted murine melanoma tumors and the expression of vegf and tnf within these tumors.Treatment with sp1 decoy odns, but not their mutated form, led to a significant increase(P=0.041)Of the tumor necrotic area, as evaluated morphometrically.Tumor necrosis was associated with a significant decrease of microvascular density(P=0.012)And relative vascular area(P=0.026), as determined by counting CD34positive vascular structures within the tumor microenvironment of Sp1 decoy ODNs and control ODNtreated tumors.Rtpcr experiments showed a strong decrease in the levels of vegf188 and vegf164 isoforms and a moderate decrease of tnf in sp1 decoytreated tumors.Taken together, our results indicate that sp1 decoy odns may inhibit angiogenesis by affecting the gene expression of key players in angiogenesis such as tnf and vegf.These findings indicate that sp1 decoy odns may be a potential new therapeutic tool in antiangiogenic therapy.